Exploring the relationships between physiochemical properties of nanoparticles and cell damage to combat cancer growth using simple periodic table-based descriptors

• Joyita Roy and
• Kunal Roy

Beilstein J. Nanotechnol. 2024, 15, 297–309, doi:10.3762/bjnano.15.27

• was obtained from Cao et al. [26], where the zeta potential of MeOx NPs was measured in a cell culture of 20% fetal bovine complete medium. Dataset II was taken from Toropova et al. [27], where cell damage measurement was performed based on the uptake of propidium iodide (PI). The dataset is related

Ion beam processing of DNA origami nanostructures

• Leo Sala,
• Agnes Zerolová,
• Violaine Vizcaino,
• Alain Mery,
• Alicja Domaracka,
• Hermann Rothard,
• Philippe Boduch,
• Dominik Pinkas and
• Jaroslav Kocišek

Beilstein J. Nanotechnol. 2024, 15, 207–214, doi:10.3762/bjnano.15.20

• 1013 ions·cm−2. Control samples were adhered at the back of the aluminum plate, in a region not exposed to the ion beam. For the high-energy irradiation, a 60 MeV/u 56Fe25+ beam was used. Samples were irradiated in air on the outer surface of a cell culture flask oriented to face the beam. Maximum flux

Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics

• Phuong-Thao Dang-Luong,
• Hong-Phuc Nguyen,
• Loc Le-Tuan,
• Xuan-Thang Cao,
• Vy Tran-Anh and
• Hieu Vu Quang

Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17

• were performed in both 0.1× PBS (13.7 mM of NaCl, 0.27 mM of KCl, 1 mM of Na2HPO4, and 0.18 mM of KH2PO4, pH 7.4) and animal cell culture media containing DMEM and 10% FBS. Scanning electron microscopy For scanning electron microscopy (SEM) experiments, 10 μL of F127-folate@NP was loaded on the silica

• additional 10 min. Cell culture The cell lines HepG2, MCF-7, 3T3, and Hek were cultured in DMEM, 10% FBS, and 1% antibiotic at 37 °C and 5% CO2. Cell uptake estimation The cells were seeded onto 6-well plates at 100,000 cells/well and were cultured overnight. On the next day, the cells were incubated with

• 0.5 mg of F127-folate@NP/Cou-6, F127@NP/Cou-6, and PVA@NP/Cou-6 in 1 mL of cell culture media for 4 h. The cells were then washed with PBS three times and trypsinized. The harvested cells were counted and 20,000 cells/well were added to a black 96-well plate in triplicates. The culture medium and

Berberine-loaded polylactic acid nanofiber scaffold as a drug delivery system: The relationship between chemical characteristics, drug-release behavior, and antibacterial efficiency

• Le Thi Le,
• Hue Thi Nguyen,
• Liem Thanh Nguyen,
• Huy Quang Tran and
• Thuy Thi Thu Nguyen

Beilstein J. Nanotechnol. 2024, 15, 71–82, doi:10.3762/bjnano.15.7

• scaffolds The cell culture medium was prepared by mixing 150 mL of Dulbecco’s modified eagle medium (DMEM) with 600 μL of 0.5 mg/mL trypsin in a Schott bottle. MA-104 cells were distributed into the wells of a 96-well plate in DMEM supplemented with 10% fetal bovine serum. The BBR NPs/PLA nanofiber scaffold

• was cut into circles with a diameter of 6 mm and sterilized with ultraviolet light for 12 h. Then these cut scaffolds were submerged in the cell culture medium in the 96-well plate and incubated for 120 h at 37 °C in an atmosphere of 5% CO2. The morphology of the cultured cells was monitored with an

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• release for three separate samples was plotted versus time. Ethanol was used in the release medium to enhance solubility and provide sink conditions for poorly water-soluble CUR. Interaction of the microparticles with cells Cell culture The effects of CUR-HSA-MPs on cell cytotoxicity were studied on human

• hepatocellular carcinoma (Huh-7) and human breast adenocarcinoma (MCF-7) cell lines using the colorimetric MTT assay. Human dermal fibroblasts (HDFB) and human cholangiocyte (MMNK-1) cell lines were evaluated as controls for cytotoxicity. Briefly, the cell lines were cultured in cell culture medium containing

• FBS (10%) and penicillin/streptomycin (1%) at 37 °C in a CO2 incubator (95% relative humidity, 5% CO2). Huh-7, HDFB, and MMNK-1 cells were cultured in DMEM, and MCF-7 cells were cultured in RPMI cell culture medium. The cells were then seeded to grow 24 h prior to treatment at a density of 5 × 103

Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes

• Brunno R. F. Verçoza,
• Robson R. Bernardo,
• Luiz Augusto S. de Oliveira and
• Juliany C. F. Rodrigues

Beilstein J. Nanotechnol. 2023, 14, 893–903, doi:10.3762/bjnano.14.73

• maintenance of Leishmania amazonensis species in Balb/C mice, the protocol number was UFRJ/CCS-142/21. Furthermore, all animals received human care according to the guide published by the Brazilian Society of Zootechnics of Laboratory and Council National Control of Animal Experimentation. Cell culture The

Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies

• Giuliana Muraca,
• María Esperanza Ruiz,
• Rocío C. Gambaro,
• Sebastián Scioli-Montoto,
• María Laura Sbaraglini,
• Gisel Padula,
• José Sebastián Cisneros,
• Cecilia Yamil Chain,
• Vera A. Álvarez,
• Cristián Huck-Iriart,
• Guillermo R. Castro,
• María Belén Piñero,
• Matias Ildebrando Marchetto,
• Catalina Alba Soto,
• Germán A. Islan and
• Alan Talevi

Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66

• incubation with the formulation of nanoparticles or the free drug. Hemolytic effect Hemolysis was assessed on 3 mL of a freshly drawn heparinized suspension of fresh human blood placed on a 6-well cell culture plate. Increasing concentrations of freshly prepared dilutions of the free drug and NLC-BNZ (1, 5

Quercetin- and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles: one-pot synthesis, characterization, and anticancer and antibacterial activities

• Akif Hakan Kurt,
• Elif Berna Olutas,
• Fatma Avcioglu,
• Hamza Karakuş,
• Mehmet Ali Sungur,
• Cansu Kara Oztabag and
• Muhammet Yıldırım

Beilstein J. Nanotechnol. 2023, 14, 362–376, doi:10.3762/bjnano.14.31

• sample was recorded at 762 nm. During the measurements, distilled water was used as a reference. Cell culture Human brain glioma (U-118 MG) and human retinal pigment epithelium (ARPE-19) cell lines were purchased from ATCC (NY, USA) and cultured in 10% (v/v) FBS (Gibco; Thermo Scientific, USA) and 1% (v

• comparable to that in the healthy ARPE-19 cells, suggesting that the effect may not be tissue specific. Another issue that may affect the results of biological applications of NPs in general and should be considered is how the colloidal distribution and stability of the NPs are affected in the cell culture

• in the microscope images of Ch/Q- and Ch/CA-Ag NPs in cell medium (see Supporting Information File 1, Figure S1), the stability of the synthesized NPs and how the size changes in cell culture medium compared to water were also evaluated by UV–vis and zeta potential measurements. An increase in the

The steep road to nonviral nanomedicines: Frequent challenges and culprits in designing nanoparticles for gene therapy

• Yao Yao,
• Yeongun Ko,
• Grant Grasman,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2023, 14, 351–361, doi:10.3762/bjnano.14.30

• be reached, the identification of structure–function and material–biological relations of NPs could be dramatically accelerated. Prevalence of reporting imaging and (or) flow cytometry techniques, protein corona, 3D cell culture model(s), and serum content during nanoparticle incubation with cells in

• investigating NP cellular uptake and (or) transfection. (c) 5-year prevalence of describing or characterizing protein corona in the manuscript. (d) 5-year prevalence of employing 3D cell culture models (e.g., spheroids or organoids). (e) Comparisons of serum content used during nanoparticle incubation with

Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer

• Filip Gorachinov,
• Fatima Mraiche,
• Diala Alhaj Moustafa,
• Ola Hishari,
• Yomna Ismail,
• Jensa Joseph,
• Maja Simonoska Crcarevska,
• Marija Glavas Dodov,
• Nikola Geskovski and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23

• toxicological profiles [134]. A systematic approach in synthesizing statistical copolymer libraries, fine-tuning nanoparticle biointeractions, and polymer bioresponsiveness, hand in hand with cell culture experiments for fast screening and dynamic cell culture models, may greatly improve the successful outcomes

In search of cytotoxic selectivity on cancer cells with biogenically synthesized Ag/AgCl nanoparticles

• Mitzi J. Ramírez-Hernández,
• Mario Valera-Zaragoza,
• Omar Viñas-Bravo,
• Ariana A. Huerta-Heredia,
• Miguel A. Peña-Rico,
• Erick A. Juarez-Arellano,
• David Paniagua-Vega,
• Eduardo Ramírez-Vargas and
• Saúl Sánchez-Valdes

Beilstein J. Nanotechnol. 2022, 13, 1505–1519, doi:10.3762/bjnano.13.124

• thermal analyzer, with a heating rate of 20 °C/min, under a nitrogen atmosphere, and a temperature range of 30–800 °C. To carry out XRD, EDX, FTIR, and TGA analyses, the pineapple peel extract and the reaction products were previously dried at 110 °C for 24 h. Cell culture In a similar manner to [51], the

Hydroxyapatite–bioglass nanocomposites: Structural, mechanical, and biological aspects

• Olga Shikimaka,
• Mihaela Bivol,
• Bogdan A. Sava,
• Marius Dumitru,
• Christu Tardei,
• Beatrice G. Sbarcea,
• Daria Grabco,
• Constantin Pyrtsac,
• Daria Topal,
• Andrian Prisacaru,
• Vitalie Cobzac and
• Viorel Nacu

Beilstein J. Nanotechnol. 2022, 13, 1490–1504, doi:10.3762/bjnano.13.123

• at −80 °C in FBS (Lonza, Belgium) with 10% DMSO (Alchimia, Italy). The ethical approval, as well as the protocol used for the isolation of the mesenchymal stem cells is described in the Annex. After thawing, cells from the third passage were cultured in DMEM/Ham F12 (Sigma, Germany) cell culture

• , USA) working solution was prepared from 0.5% (w/v) MTT stock solution in DMEM/Ham F12 cell culture media. After removing the samples and cell culture media, MTT working solution was poured in the plates followed by incubation of the plates at 37 °C, 5% CO2 for 2 h, then the formazan crystals were

• (HiMedia, India) and pipetted several times to generate a single-cell suspension. After repeated centrifugation, the cells were resuspended in mesenchymal stem cell expansion medium and transferred into a 25 cm2 cell culture flask and incubated at 37 °C in 5%CO2 humid environment. In the first passage

Facile preparation of Au- and BODIPY-grafted lipid nanoparticles for synergized photothermal therapy

• Yuran Wang,
• Xudong Li,
• Haijun Chen and
• Yu Gao

Beilstein J. Nanotechnol. 2022, 13, 1432–1444, doi:10.3762/bjnano.13.118

• (FOTRIC 225 s, USA). Cell culture Murine breast cancer 4T1 cell line was purchased from Cell Resource Center of Shanghai Institute for Biological Sciences (Chinese Academy of Sciences, Shanghai, China). 4T1 cells were cultured in RPMI-1640 medium containing 100 μg/mL streptomycin, 100 U/mL penicillin, and

Orally administered docetaxel-loaded chitosan-decorated cationic PLGA nanoparticles for intestinal tumors: formulation, comprehensive in vitro characterization, and release kinetics

• Sedat Ünal,
• Osman Doğan and
• Yeşim Aktaş

Beilstein J. Nanotechnol. 2022, 13, 1393–1407, doi:10.3762/bjnano.13.115

• treatment of colon tumors, to provide higher drug concentration in the tumoral region in the colon, to reduce systemic side effects compared to parenteral administrations, and to alleviate colon cancer. In vitro characterization, permeability studies, in vitro cell culture studies, and comprehensive release

• [15]. Manca et al. stated that using CS as coating material significantly increased the mucoadhesive activity of the formulations by positively changing the surface charge of the NPs [54]. Cell culture models are not precise enough to evaluate the interaction between NPs and mucus layer. Since the

• of CS on the nanoparticle surface led to a slower release. All results together indicate a release based on Fickian diffusion. In vitro cell culture studies Antiproliferative effect of DCX loaded PLGA nanoparticles against HT-29 cell line The antiproliferative activity of DCX-loaded and blank NPs was

Recent advances in green carbon dots (2015–2022): synthesis, metal ion sensing, and biological applications

• Aisha Kanwal,
• Naheed Bibi,
• Sajjad Hyder,
• Arif Muhammad,
• Hao Ren,
• Jiangtao Liu and
• Zhongli Lei

Beilstein J. Nanotechnol. 2022, 13, 1068–1107, doi:10.3762/bjnano.13.93

Bioselectivity of silk protein-based materials and their bio-inspired applications

• Hendrik Bargel,
• Vanessa T. Trossmann,
• Christoph Sommer and
• Thomas Scheibel

Beilstein J. Nanotechnol. 2022, 13, 902–921, doi:10.3762/bjnano.13.81

• ]. Cell culture studies on film surfaces using Balb 3T3 fibroblasts [148], neonatal rat cardiomyocytes [162], human induced pluripotent stem cell (hiPSC) derived cardiomyocytes [150], keratinocytes, myoblasts, or neuronal cells [163] revealed that negatively eADF4(C16) and uncharged eADF4(Ω16) surfaces do

• a residue substitution of aspartate with glutamate thus rendering an identical charge distribution, was applied as a control. Cell culture studies using Balb 3T3 fibroblasts revealed that the RGD peptide enhanced primary cell attachment with increased spread morphology and proliferation on films

• , RGE, IKVAV, and YIGSR sequences at their N-terminal end. Cell culture analysis using primary cells (fibroblasts, keratinocytes, endothelial, and Schwann cells) revealed that these peptide tags had a clear positive impact on early cell attachment and spreading (Figure 3B). Especially, adhesion and

Gelatin nanoparticles with tunable mechanical properties: effect of crosslinking time and loading

• Agnes-Valencia Weiss,
• Daniel Schorr,
• Julia K. Metz,
• Metin Yildirim,
• Saeed Ahmad Khan and
• Marc Schneider

Beilstein J. Nanotechnol. 2022, 13, 778–787, doi:10.3762/bjnano.13.68

• system is usually tested intensively in vitro using cell culture-based assays before application in in vivo studies. Depending on the particle material, the mechanical properties will be influenced, also modulating potentially the particle–cell interaction. For polymeric particles, a higher uptake for

Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy

• Xiaoqiong Tang,
• Yan Zhang,
• Jiangbing Mao,
• Yuhua Wang,
• Zhenghong Zhang,
• Zhengchao Wang and
• Hongqin Yang

Beilstein J. Nanotechnol. 2022, 13, 560–569, doi:10.3762/bjnano.13.47

• study may provide a new approach to investigate the migration mechanism of PCa cells in addition to cytoskeleton-mediated migration of PCa cells, which can be useful for cancer therapeutic drug screening and development. Experimental Cell culture PZ-HPV-7 normal human prostate epithelial cells and PC-3

• main manuscript as well as in the Supporting Information. The values are presented as mean ± standard deviation of multiple measurements and statistical significance was considered at P < 0.05. Schematic diagram of polyacrylamide hydrogel substrate preparation and cell culture process. Effect of

Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

• Gufeng Li,
• Shaoqing Li,
• Rui Wang,
• Min Yang,
• Lizhu Zhang,
• Yanli Zhang,
• Wenrong Yang and
• Hongbin Wang

Beilstein J. Nanotechnol. 2022, 13, 549–559, doi:10.3762/bjnano.13.46

• carried out in triplicate. Cell culture and imaging of intracellular molecular release HeLa cells were incubated in Dulbecco's modified Eagle medium (DMEM) (the density is about 2 × 104 cells per well) at 37 °C in a 5% CO2 for 48 h. After adding 100 μL of GSH-Rh6G2 and GNPs-GSH-Rh6G2 for 1 h, the cells

Ethosomal (−)-epigallocatechin-3-gallate as a novel approach to enhance antioxidant, anti-collagenase and anti-elastase effects

• Çiğdem Yücel,
• Gökçe Şeker Karatoprak,
• Sena Yalçıntaş and
• Tuğba Eren Böncü

Beilstein J. Nanotechnol. 2022, 13, 491–502, doi:10.3762/bjnano.13.41

• (−)-Epigallocatechin-3-gallate, 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), SPC, trypsin-EDTA solution, dimethyl sulfoxide (DMSO) for cell culture, and penicillin/streptomycin solution were purchased from Sigma, USA. Carbopol 980 was purchased from the Abdi İbrahim Pharmaceutical, Industry and

• Biochrom, Germany. Cell culture flasks and plates were purchased from Corning®. Cedex. Smart Slides and Trypan Blue solution were purchased from Roche (Switzerland). Mouse collagenase and elastase enzyme ELISA kits were obtained from Sunredbio, Shanghai. HPLC assay The quantitative determination (i.e., the

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

• Zahra Nabizadeh,
• Mahmoud Nasrollahzadeh,
• Hamed Daemi,
• Mohamadreza Baghaban Eslaminejad,
• Ali Akbar Shabani,
• Mehdi Dadashpour,
• Majid Mirmohammadkhani and
• Davood Nasrabadi

Beilstein J. Nanotechnol. 2022, 13, 363–389, doi:10.3762/bjnano.13.31

• Biological cues in cartilage TE Natural ECM provides a desirable microenvironment containing biologically important motifs (Table 2) for cell attachment, spread, migration, proliferation, and differentiation [149]. This microenvironment is inevitably disregarded during the in vitro cell culture and expansion

• . Cell culture in 3D matrices, unlike 2D cultures, provides the opportunity to include and recruit the necessary bioactive molecules for proper cell function [177]. In addition to biopolymers, which provide structural integrity and stability, ECM consists of protein motifs and full-length proteins

Photothermal ablation of murine melanomas by Fe₃O₄ nanoparticle clusters

• Xue Wang,
• Lili Xuan and
• Ying Pan

Beilstein J. Nanotechnol. 2022, 13, 255–264, doi:10.3762/bjnano.13.20

• line was provided by the Core Laboratory at China–Japan Union Hospital of Jilin University. Use of this cell line was approved by the Animal Experimental Ethical Inspection Committee at Jilin University (Permit no.: 201802034). Cell culture was performed as previously described [15]. After reaching 70

• ) Magnetic hysteresis curves of individual Fe3O4 nanoparticles as well as NPCs. The insert shows aggregation of NPCs dispersed in aqueous solution by a magnet. (d) DLS characterization of NPCs dispersed in cell culture medium. (e) DLS results of NPCs suspension after five heating/cooling cycles. Photothermal

Bacterial safety study of the production process of hemoglobin-based oxygen carriers

• Axel Steffen,
• Yu Xiong,
• Radostina Georgieva,
• Ulrich Kalus and
• Hans Bäumler

Beilstein J. Nanotechnol. 2022, 13, 114–126, doi:10.3762/bjnano.13.8

• with doxorubicin, a cytostatic drug used in chemotherapy for cancer treatment. These particles showed higher efficacy in inhibiting metabolic activity in cell culture in comparison to free doxorubicin [8]. To be used as an artificial oxygen carrier, hemoglobin is isolated from bovine blood. Compared to

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• ) in HBG at 25 °C. All measurements were performed in triplicate. Cell culture and cytotoxicity assay Dose-dependent antiproliferative effects of free bPEI, SPION@bPEI, and SPION@bPEI/PIC were tested on HeLa cells (cervical cancer cell line). The antiproliferative effect of SPION@bPEI-Erb and SPION

Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy

• Xiaoteng Liang,
• Shuai Liu,
• Xiuchao Wang,
• Dan Xia and
• Qiang Li

Beilstein J. Nanotechnol. 2021, 12, 1372–1379, doi:10.3762/bjnano.12.101

• Shijiazhuang Hongwei Biotechnology Co., Ltd. AF488-WGA and Hoechst 33342 were purchased from Thermo Fisher Scientific. All medicals and reagents were used without further treatment. Cell culture HPDE6-C7, AsPc-1 and BxPC-3 cells were cultured in RPMI-1640 with 10% fetal bovine serum and 1% double antibody. MIA